GATK4 pipeline using Galaxy is a scientific workflow especially for the students not having expertise in Linux operating system and other command-line interfaces (CLI) for NGS data analysis. This course will cater to undergraduate and postgraduate students from a wide range of life sciences disciplines to work independently from data retrieval to publishing their articles. A plethora of NGS data tools are available on a single platform, otherwise its very difficult to manage all the latest releases of software/tools on your personal computers, if yes, we have other space, storage and expertise issues to work on CLI being a biology student at an individual level. No doubt there are cloud computing and commercial high performance computing cluster facilities are available in the world but this course will provide you the insight that how to analyze multi-omics data with limited financial resources without having much expertise in programming and knowhow to computer languages.